研究業績

荒木喜美

原著論文

  1. Ariyasu, D., Kubo, E., Higa, D., Shibata, S., Takaoka, Y, Sugimoto, M., Imaizumi, K., Hasegawa, T. and Araki, K. Decreased Activity of the Ghrhr and Gh Promoters Causes Dominantly Inherited GH Deficiency in Humanized GH1 Mouse Models. Endocrinology. In press.2019
  2. Li, X., Lyu, Y., Shen, J., Mu, Y., Qiang, L., Liu, L., Araki, K., Imbimbo, B.P., Yamamura, K., Jin, S. and Li, Z. Amyloid deposition in a mouse model humanized at the transthyretin and retinol-binding protein 4 loci. Lab Invest. 98: 512-524, 2018.
  3. Kim, Y. J., Osborn, D. P., Lee, J. Y., Araki, M., Araki, K., Mohun, T., Kansakoski, J., Brandstack, N., Kim, H. T., Miralles, F., Kim, C. H., Brown, N. A., Kim, H. G., Martinez-Barbera, J. P., Ataliotis, P., Raivio, T., Layman, L. C. and Kim, S. H. WDR11-mediated Hedgehog signalling defects underlie a new ciliopathy related to Kallmann syndrome. EMBO Rep. 19: 269-289, 2017.
  4. Honda, I., Araki, K., Honda, S., Soeda, F., Shin, M. C., Misumi, S., Yamamura, K. I. and Takahama, K. Deletion of GIRK2 subunit containing GIRK channels of neurons expressing dopamine transporter decrease immobility time on forced swimming in mice. Neurosci. Lett. 665: 140-146, 2017.
  5. Kurogi, S., Sekimoto, T., Funamoto, T., Ota, T., Nakamura, S., Nagai, T., Nakahara, M., Yoshinobu, K., Araki, K., Araki, M. and Chosa, E. Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models. Sci. Rep. 7: 40692, 2017.
  6. Tanaka, K., Kim, S. E., Yano, H., Matsumoto, G., Ohuchida, R., Ishikura, Y., Araki, M., Araki, K., Park, S., Komatsu, T., Hayashi, H., Ikematsu, K., Hirano, A., Martin, P., Shimokawa, I. and Mori, R. MiR-142 Is Required for Staphylococcus aureus Clearance at Skin Wound Sites via Small GTPase-Mediated Regulation of the Neutrophil Actin Cytoskeleton. J. Invest. Dermatol. 137: 931-940, 2017.
  7. Liu, L., Suzuki, T., Shen, J., Wakana, S., Araki, K., Yamamura, K., Lei, L. and Li, Z. Rescue of retinal morphology and function in a humanized mouse at the mouse retinol-binding protein locus. Lab. Invest. 97: 395-408, 2017.
  8. Sakata, K., Araki, K., Nakano, H., Komazawa-Sakon, S., Lee, G. E., Hashimoto, D., Suzuki, C., Uchiyama, Y., Notohara, K., Gukovskaya, A. S., Gukovsky, I., Yamamura, K., Baba, H. and Ohmuraya, M. Novel method to rescue a lethal phenotype through integration of target gene onto the X-chromosome. Sci. Rep. 6: 37200, 2016.
  9. Kacso, G., Ravasz, D., Doczi, J., Nemeth, B., Madgar, O., Saada, A., Ilin, P., Miller, C., Ostergaard, E., Iordanov, I., Adams, D., Vargedo, Z., Araki, M., Araki, K., Nakahara, M., Ito, H., Gal, A., Molnar, M., Nagy, Z., Patocs, A., Adam-Vizi, V., and Chinopoulos C. Two transgenic mouse models for beta subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations. Biochemical J. 473: 3463-3485, 2016.
  10. Vanhoutteghem, A., Delhomme, B., Herve, F., Nondier, I., Petit, J. M., Araki, M., Araki, K. and Djian, P. The importance of basonuclin 2 in adult mice and its relation to basonuclin 1. Mech. Dev. 140: 53-73, 2016.
  11. Shen, J., Shi, D., Suzuki, T., Xia, Z., Zhang, H., Araki, K., Wakana, S., Takeda, N., Yamamura, K., Jin, S. and Li, Z. Severe ocular phenotypes in Rbp4-deficient mice in the C57BL/6 genetic background. Lab. Invest. 96: 680-91, 2016.
  12. Taki, K., Ohmuraya, M., Tanji, E., Komatsu, H., Hashimoto, D., Semba, K., Araki, K., Kawaguchi, Y., Baba, H. and Furukawa, T. GNAS and Kras cooperate to promote murine pancreatic tumorigenesis recapitulating human intraductal papillary mucinous neoplasm. Oncogene 35: 2407-12, 2015.
  13. Chen, J., Fujino, R., Zhao, R., Semba, U., Araki, K. and Yamamoto, T. Role of blood ribosomal protein S19 in coagulum resorption: a study using Gln137Glu-ribosomal protein S19 gene knock-in mouse. Pathol. Int. 64, 543-50, 2014.
  14. Tomonoh, Y., Deshimaru, M., Araki, K., Miyazaki, Y., Arasaki, T., Tanaka, Y., Kitamura, H., Mori, F., Wakabayashi, K., Yamashita, S., Saito, R., Itoh, M., Uchida, T., Yamada, J., Migita, K., Ueno, S., Kitaura, H., Kakita, A., Lossin, C., Takano, Y., Hirose, S. The kick-in system: a novel rapid knock-in strategy. PLoS One. 9: e88549, 2014.
  15. Araki, M., Nakahara, M., Muta, M., Itou, M., Yanai, C., Yamazoe, F., Miyake, M., Morita, A., Araki, M., Okamoto, Y., Nakagata, M., Yoshinobu, K., Yamamura, K. and Araki, K. Database for exchangeable gene trap clones: Pathway and gene ontology analysis of exchangeable gene trap clone mouse lines. Develop. Growth Differ. 56: 161-74.  2014.
  16. Nakahara, M., Tateyama, H., Araki, M., Nakagata, M., Yamamura and Araki, K. Gene trap mutagenesis using Mol/MSM-1 ES cells established from MSM/Ms strain. Mamm. Genome.24: 228-239. 2013.
  17. Semba, K., Araki, K., Matsumoto, K., Suda, H., Ando, T., Sei, A., Mizuta, H., Takagi, K., Nakahara, M., Muta, M., Yamada, G., Nakagata, N., Iida, A., Ikegawa, S., Nakamura, Y., Araki, M., Abe, K. and Yamamura, K. Ectopic Expression of Ptf1a Induces Spinal Defects, Urogenital Defects, and Anorectal Malformations in Danforth’s Short Tail Mice. Plos Genet. 9(2): e1003204, 2013.
  18. Araki, K., Okada, Y., Araki, M. and Yamamura, K. Comparative analysis of right-element mutant lox sites on recombination efficiency in embryonic stem cells. BMC Biotechnol. 10: 29, 2010.
  19. Araki, K., Takeda, N., Yoshiki, A., Obata, Y., Nakagata, N., Shiroishi, T., Moriwaki, K. and Yamamura, K. Establishment of germline-competent embryonic stem cell lines from the MSM/Ms strain. Mamm Genome, 20(1): 14-20, 2009.
  20. Chen, H., Li, Z., Haruna, K., Li, Z., Li, Z., Semba, K., Araki, M., Yamamura, K. and Araki, K. Early pre-implantation lethality in mice carrying truncated mutation in the RNA polymerase 1-2 gene. Biochem. Biophys. Res. Commun. 365(4): 636-42, 2008.
  21. Yamamura, K. and Araki, K. Gene trap mutagenesis in mice: New perspectives and tools in cancer research. Cancer Sci. 99(1): 1-6, 2008.
  22. Hoshii, T., Takeo, T., Nakagata, N., Takeya, M., Araki, K. and Yamamura, K. LGR4 regulates the postnatal development and integrity of male reproductive tracts in mice. Biol. Reprod.76(2): 303-13, 2007.
  23. Araki, K., Araki, M. and Yamamura, K. Negative selection with the Diphtheria toxin A fragment gene improves frequency of Cre-mediated cassette exchange in ES cells. J. Biochem. 140(6): 793-8, 2006.
  24. Miura, K., Yoshinobu, K., Imaizumi, T., Haruna, K., Miyamoto, Y., Yoneda, Y., Nakagata, N., Araki, M., Miyakawa, T., Yamamura, K. and Araki, K. Impaired expression of Importin/karyopherin β1 leads to post-implantation lethality. Biochem. Biophys. Res.Commun. 341(1): 132-8, 2006.
  25. Taniwaki, T., Haruna, K., Nakamura, H., Sekimoto, T., Oike, Y., Imaizumi, T., Saito, F., Muta, M., Soejima, Y., Utoh, A., Nakagata, N., Araki, M., Yamamura, K. and Araki, K. Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-bgeo cassette. Dev. Growth Differ. 47(3):163-72, 2005.
  26. Hino, H., Araki, K., Uyama, E., Takeya, M., Araki, M., Yoshinobu, K., Miike, K., Kawazoe, Y., Maeda, Y., Uchino, M. and Yamamura, K. Myopathy phenotype in transgenic mice expressing mutated PABPN1 as a model of oculopharyngeal muscular dystrophy. Hum. Mol. Genet. 13(2): 181-90, 2004.
  27. Araki, K., Araki, M. and Yamamura, K. Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites. Nucleic Acids Res. 30(19): e103, 2002.
  28. Araki, K., Imaizumi, T., Sekimoto, T., Yoshinobu, K., Yoshimuta, J., Akizuki, M., Miura, K., Araki, M. and Yamamura, K. Exchangeable gene trap using the Cre/mutated lox system. Cell Mol. Biol. 45(5): 737-50, 1999.
  29. Oike, Y., Hata, A., Mamiya, T., Kaname, T., Noda, Y., Suzuki, M., Yasue, H., Nabeshima, T., Araki, K. and Yamamura, K. Truncated CBP protein leads to classical Rubinstein-Taybi syndrome phenotypes in mice: Implication of a dominant negative mechanism. Human Mol. Genet. 8(3): 387-96, 1999.
  30. Araki, K., Imaizumi, T., Okuyama, K., Oike,Y. and Yamamura, K. Efficiency of recombination by Cre transient expression in embryonic stem cells: Comparison of various promoters. J. Biochem. 122(5): 977-82, 1997.
  31. Araki, K., Araki, M. and Yamamura,K. Targeted integration of DNA using mutant lox sites in embryonic stem cells. Nucleic Acids Res. 25(4): 868-72, 1997.
  32. Rodriguez, I., Araki, K., Karim, K., Martinou, J.-C. and Vassalli, P. Mouse vaginal opening is an apoptosis-dependent process which can be prevented by the overexpression of Bcl2. Dev. Biol. 184(1): 115-21, 1997.
  33. Biancone, L., Araki, M., Araki, K., Vassalli,P. and Stamenkovic, I. Reduction of tumor metastasis by expression of E-selectin in vivo. J. Exp. Med. 183(2): 581-7, 1996.
  34. Araki, K., Araki, M., Miyazaki, J. and Vassalli, P. Site specific recombination of transgene in fertilized eggs by transient expression of Cre recombinase. Proc. Natl. Acad. Sci., USA. 92(1): 160-4, 1995.
  35. Araki, K., Miyazaki, J., Hino, O., Tomita, N., Chisaka, O., Matsubara, K. and Yamamura, K. Expression and replication of hepatitis B virus genome in transgenic mice. Proc. Nathl. Acad. Sci. USA. 86(1): 207-11, 1989.

学会発表

  1. 荒木喜美, 中原 舞, 牟田真由美, 荒木正健, 山村研一：単独では高寄与率のキメラを得ることが困難なES細胞からヘルパーES細胞を用いることにより生殖系列キメラを得る手法の開発, 日本遺伝学会第84会大会, 2012.9.24-9.26, 福岡（九州大学医学部）
  2. 荒木喜美, 仙波 圭, 中原 舞, 牟田真由美, 阿部訓也, 荒木正健, 山村研一: Danforth’s short tail(Sd)変異の原因はPtf1aの異所性発現である, 第35回日本分子生物学会年会, 2012.12.11-12.14, 福岡（福岡国際会議場）
  3. 中原 舞, 荒木喜美, 荒木正健, 作村由美, 鶴田優子, 牟田真由美, 舘山浩紀, 山村研一：MSM/Ms系統由来. ES細胞を用いた遺伝子トラップクローンの解析. 日本遺伝学会第83回大会, 2011. 9.20-23, 京都（京都大学農学部・農学研究科）
  4. 荒木喜美, 大村谷昌樹, 荒木正健, 山村研一: 部位特異的組換えシステムを用いたマウスゲノムエンジニアリング. 日本遺伝学会第83回大会, 2011.9.20-23, 京都（京都大学農学部・農学研究科）（招待講演）
  5. 荒木喜美 : ES細胞における部位特異的組換えシステムの開発とそれを用いた可変型遺伝子トラップデータベースの構築. 第25回モロシヌス研究会, 2011. 7.8-9, 新潟（当間高原リゾート「ベルナティオ」）【森脇和郎賞受賞講演】
  6. 荒木喜美：MSM由来ES細胞を用いた遺伝子操作マウスの作出と解析「シンポジウムⅠ：野生マウスが拓く新しい実験動物学の地平」. 第58回日本実験動物学会総会, 2011. 5.25-27, 東京（タワーホール船堀）（招待講演）
  7. 荒木喜美, 大村谷昌樹, 荒木正健, 山村研一: 新しい部位特異的組換えシステムDre/roxを用いたES細胞及びマウス個体での組換え効率の検討. 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会, 2010. 12.7-10, 兵庫(神戸ポートアイランド)
  8. Araki, M., Araki, K., Tsuruta, Y., Sakumura, Y., Muta, M., Keita, T., Koga, N., Egami, T., Kimachi, Y., Yoshinobu, K., Yamamura, K.: Database for the Exchangeable Gene Trap Clones (EGTC). The 4th Asian Mouse Mutagenesis and Resource Association. Dec. 17-18. 2009, Kumamoto (Japan)
  9. 荒木喜美, 平山知美, 作村由美, 荒木正健, 山村研一: 可変型遺伝子トラップクローンを利用したトランスジェニックマウス作製システムの構築. 第56回日本実験動物学会総会, 2009. 5.14-16, 埼玉（大宮ソニックシティ）
  10. 荒木喜美，陳輝，湊理恵，鶴田優子，牟田真由美，吉信公美子，吉木淳，荒木正健，山村研一：可変型遺伝子トラップクローンを用いたCre-driverマウスの効率的作製. BMB2008（第31回日本分子生物学会年会・第81回日本生化学会大会合同大会）, 2008. 12.9-12, 兵庫（神戸国際会議場）
  11. 荒木喜美，竹田直樹，吉木淳，山田源，中潟直己，城石俊彦，森脇和郎，山村研一：MSM/Ms及びJF1/Ms系統からのES細胞株の樹立. 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会, 2007. 12.11-15，神奈川（パシフィコ横浜）
  12. Araki, K., Takeda, N., Yoshiki, A., Yamada,G., Nakagata, N., Shiroishi, T,., Moriwaki, K. and Yamamura, K.: ESTABLISHMENT OF EMBRYONIC STEM CELL LINES DERIVED FROM MSM/MS STRAIN ORIGINATED FROM MUS MUSCULUS MOLOSSINUS., 21st International Mammalian Genome Conference IMGC2007, Oct.28-Nov.1. 2007, Kyoto(Japan)
  13. Araki, M., Araki, K., Yoshinobu, K., Haruna, K., Sakumura, Y. and Yamamura,K.: EGTC, DATABASE FOR THE EXCHANGEABLE GENE TRAP CLONES; RESOURCE OF MOUSE AND ES CELL LINES FOR THE FUNCTIONAL ABALYSIS OF THE MOUSE GENOME., 21st International Mammalian Genome Conference IMGC2007, Oct.28-Nov.1. 2007, Kyoto(Japan)
  14. 荒木喜美，岡田由香，荒木正健，山村研一：ES細胞においてlox71部位に対して良い組換え効率を示す新たなRE変異loxの探索(Comparative analysis of newly identified right-element mutant lox sites on the efficiency of lox71 site-directed integration in ES cells). 第40回日本発生生物学会・第50回日本細胞生物学会合同大会，2007. 5.28-30, 福岡（福岡国際会議場）
  15. 荒木喜美：我が国独自のノックアウトマウスプロジェクト（シンポジウム）. 第54回日本実験動物学会総会. 2007. 5.23-25, 東京（タワーホール船堀）
  16. Araki, K., Araki, M., Yamamura, K.: Negative selection with the DT-A gene improves frequency of Cre-mediated cassette exchange in ES cells. 日本発生生物学会第39回大会，2006. 5.31．-6.3，広島（広島国際会議場）
  17. Araki, K.: Alteration of a trap allele into four different alleles by using Cre/mutant lox system. 2004 International Gene Trap Workshop, 7.1-7.2. 2004, Cambridge(UK)
  18. Araki, K., Araki, M., Yamamura, K.: Efficient site cassette exchange using Cre/mutant-lox sysutem in ES cells, Frontiers in Genome Engineering Building a Better Mouse, 6.6-9. 2004, Nashville(U.S.A.)（Invited Talk）
  19. Araki, K.: Efficient gene replacement in exchangeable gene trap lines. 2nd International Gene Trap Workshop, 5.30-6.1. 2002, Frankfurt am Main(Germany)
  20. Araki, K., Araki, M., Imaizumi, T., Sekimoto, T., Yamamura, K.: Exchangeable gene trap using Cre/mutated lox system. Mouse Molecular Genetics Meeting, 9.1-5. 1999, Heidelberg(Germany)
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